FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323579_209_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323579_209_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11561514
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT14500.011.18195349
CGGTACA17900.010.40639661
GTAGTCG9850.09.80427957
GGCGGTA19750.09.25555859
GATTCCG16900.08.9802852
TTCTCGA16350.08.86017547
TCTCGAT15600.08.62381948
GTCTAAG22000.08.46698644
TAGTCGC11600.08.32522258
GCGTATA6705.901038E-78.23682239
CGCGGTA6756.6082066E-78.17559617
TCGATTC19550.08.11706250
GTCGAAT5201.18364376E-48.0530491
GCGGTAC22850.07.84921160
AGTCGCC13100.07.63520359
GTTTAAT53400.07.58052351
ATTCCGT18300.07.53926653
GCGTAGT10556.348273E-107.51883318
CGCTAGT5552.8728304E-47.45911533
TGCGTAG12609.094947E-127.39088517