FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_301_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_301_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11922712
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGGT16500.013.4983911
CCCGTAT10600.012.4756661
GTCGGTT11600.012.0001871
CCCGAAT14100.011.3533691
GTCGCTT16600.011.3206581
GTCGCAT9550.011.2965111
GTCGGCT18550.011.2562391
GGCGTTT19550.011.2144971
GTCGGGA17300.010.46027851
GTCGTAT12700.010.412761
GTCCGTT15450.09.9108331
TCGACCG5959.145879E-99.85614331
GTCCAGT60700.09.8037771
GCCAGAT49150.09.7710571
GTCGAAA6909.0221874E-109.5827581
GTCGTTT20500.09.5064891
GTTCGTT19050.09.499361
GTCGAGT10650.09.4762031
GGCGTAA11050.09.4481111
GTCGGAT12700.09.316681