FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_296_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_296_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10942310
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT12100.010.5408549
TCTCGAT14600.08.73590948
GATTCCG15400.08.50644252
ATTCCGT16300.07.82530353
TCGATTC15900.07.8042850
TTCTCGA16500.07.520591347
GTCTAAG18800.07.517463744
CGGTACA17650.07.421033461
GGCGGTA18600.07.22758259
TACGCGA4850.00486106157.10770555
TTCCGAT15100.07.0766892
CGATTCC19950.06.911942551
TTTCTCG19350.06.769427346
CCGATCT15352.3646862E-116.5111774
TGGGACG34700.06.3614761
ACACACG27200.06.33703552
CTTCCGA19900.06.2396011
TCCGATC16108.0035534E-116.2126881
TAGCATA28650.06.1356299
ACACGGG28100.06.011415554