FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_296_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_296_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10942310
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTT14350.013.3292431
TCGACCG6950.011.91236131
CGACCGT8000.011.21126832
CCCGATT10750.010.9993021
GTCGGGA17050.010.6065381
CCGGAAA10950.010.4808031
GTTCGAC8000.010.34829629
GTCCGAT11900.010.2285891
CCGGTAT11250.010.2013141
CTCGTAT16400.09.9666391
TCGCGTA3854.5624227E-59.85826866
CCCGAAT14100.09.6192261
GTCCGTA14150.09.5852371
GTCGGTT9800.09.5814751
CGTATCG7951.4551915E-119.54869653
CTCGGAT19400.09.500991
CCCGGTT18100.09.4148241
GTCGTAT9750.09.2739221
CTCCGTT26850.09.0666841
CTCGGTT16000.08.9116591