FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_295_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_295_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20658370
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT24400.010.45457149
CGGTACA33150.010.08575361
GGCGGTA33050.09.8036159
GATTCCG28700.09.60938552
GTCTAAG36650.09.31115344
TTCTCGA29300.09.29393447
TCTCGAT28850.09.08096748
TCGATTC29900.08.64625650
GCGGTAC37650.08.51419160
ATTCCGT31500.08.09857653
CGCGGTA11607.2759576E-127.7925517
TAGCATA55500.07.26618969
GTTTAAT93700.07.10475641
GCGTATA13751.0913936E-117.019326739
CGGTAAT15400.06.998503719
CCGGACA40900.06.99476112
ACGGGCG49700.06.86680556
TCACCCG45500.06.74137263
TTTCTCG42250.06.690309546
CATATGC60450.06.614237312