FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_295_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_295_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20658370
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG12550.015.66780231
CGACCGT14250.014.76698732
GTTCGAC14550.013.75067729
GTCCGCT27800.012.8917281
GTCGGTT19350.011.3286531
GTCGGAT23150.011.2727271
TCGACGC19100.011.01688143
CCCGAAT22700.010.4232161
GTCGCAT16950.010.2640551
GTCGCTT29000.010.0785941
AAGTTCG19000.09.9853927
AGTTCGA20500.09.92782428
TTCGACC19950.09.85578130
GTCGAGA14800.09.6391961
GTCCGTT24600.09.6181711
GTCGTAT21550.09.5262821
GCCCGTT41000.09.2504181
GATCGCG9500.09.07804440
GCCGTAT20000.09.0467391
GTCCGGT30800.09.0377011