FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_259_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_259_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10842295
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT13900.011.15967949
CGCGGTA5653.443347E-910.4603517
GATTCCG16350.010.33185952
TCTCGAT15800.010.03586448
TCGATTC16800.09.43809950
ATTCCGT16950.09.35595753
TTCTCGA16900.09.1782947
CGGTACA18000.08.8086961
CCGGACA21450.08.51668512
CGATTCC20300.08.49125951
GGCGGTA19050.08.32336359
TCACCCG22400.08.154473
GCGTATA7001.1559732E-67.878906739
CGGTAAT8001.1464908E-77.82218819
GCGGTAC20900.07.58650160
TTTCTCG22800.07.559362446
CCCGGAC23750.07.40178711
CGTGGGT24250.07.25024257
TAGCATA29350.07.04633249
GTCTAAG20950.06.91043344