FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_259_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_259_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10842295
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG7250.014.74967831
GTCCGTT13600.013.3043361
CGACCGT8600.013.23653432
GTCGCAT8900.012.9018711
GTTCGAC8900.012.79000729
GATCGCG3701.8454739E-712.1203340
CCCGTAT10650.012.0887351
GTCGTTT19400.011.4790761
GTCCGCT15000.011.3666771
GTCCGAT11050.011.3362381
GTCGGTT10850.011.2245011
CGCTAGA3752.5650006E-611.1347051
TTCGACC9650.011.08105730
TCGACGC10050.010.64036443
GTCGGAT11850.010.5709231
GTCGAAT8600.010.5197071
GTCCGTA12100.010.3525151
CGCTAGT4107.4566087E-610.1841811
GCGATCG10200.09.80777940
GTCCGAA10300.09.7969191