FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_258_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_258_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4916560
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGC3501.9177598E-49.84927157
TAAGCGC3753.8397583E-49.19143720
GTTTAAT22350.09.1037211
CTCGATT4704.1889012E-58.80138749
GCTTATC15250.08.36414752
ACTAGGC6051.12244325E-57.97644511
ACTCACT17250.07.7917896
AACCGAT7554.3399268E-77.76232454
GAGTCGA4000.0070075047.7539344
ATTTCCG6008.745668E-57.4671316
GTTCAAT13400.07.46341561
CCTAACT16350.07.37882228
TTCGGCG6553.2259748E-57.367781638
CTAACTT17350.07.35117329
ACCCATG20800.07.12470346
CGGTACA6855.829057E-57.044675461
CGGGAAT9908.644929E-86.96689461
TCGATTC5956.4395915E-46.951860450
GGAAAAT45800.06.9273451
TCTCGAT5000.0065316966.89441948