FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_258_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_258_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4916560
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGTT3700.017.8646951
TCGACCG2705.2670657E-815.33146531
GTCCGCT5650.014.7776891
CGACCGT3152.2484528E-814.23636232
GATCGCG1750.001136292413.79916240
CGCGAGT2001.9850736E-413.7987443
GTCGAAT5300.013.7843761
CCGCATA3102.8417162E-713.3567443
CCCGTAT3405.480797E-813.301731
GTCCGTT5600.013.0459281
GTCGTAT6000.012.7560191
GTTCGAC3255.066813E-712.73587429
GTCCGAT7400.012.6933361
GTCCGAA4154.289177E-912.5743881
GTCGTTT8400.012.4246931
TCGCGAG2306.458891E-411.99927142
GTCGGGT6057.2759576E-1211.5005421
GTCGGAT6700.011.42331
CGGATCG2150.00532388511.23187538
GTCGGCT7200.011.1131981