FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_257_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_257_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24014950
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTCCG28850.08.24521752
CTCGATT26350.08.24186149
CGGTACA33700.08.08021261
TCTCGAT28600.07.59346348
GGCGGTA36650.07.43007459
TCGATTC33750.07.353624350
ATTCCGT32400.07.23541853
TTCTCGA32950.07.113444347
GTTTAAT114050.06.80452441
GCGGTAC42650.06.78884360
GTCTAAG42150.06.705958444
ATCGACT38100.06.5130346
GTTTATT150950.06.51211831
TAAATCG18000.06.50969363
GTTGAAT98750.06.49660351
GTTTTTT316850.06.4552271
CGATACG24200.06.40967929
CCGCTAG13502.4574547E-96.383301365
GTTTTAT195200.06.37878471
TACATCG45950.06.30010653