FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_257_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_257_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24014950
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG10300.016.07563831
CGACCGT11900.014.20408632
GTTCGAC14000.012.56569329
GTCGGTT20750.012.0774091
GTCGCAT17100.011.3985941
GTCGATT31300.011.009061
GTCGAAT21150.010.8615781
GTCCGGA32750.010.6279151
GTCGCTT31850.010.6003871
GTCCGTT30750.010.526821
GTCCGCT31500.010.4971741
CGATCGG14200.010.44618841
AGTTCGA19900.010.22677528
CCCGAAT28750.010.1695291
TCGACGC18150.010.07308643
CCCGTAT20150.010.0187221
GTCCAGT111050.09.7476791
GTCGTTT42700.09.700151
GTCGAGA17950.09.6953831
GTCGGAA39650.09.6562591