FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_256_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_256_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6241795
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGA2601.3569454E-411.93298756
TTACGCG3209.2486676E-49.69555255
CGAACGA4058.029836E-59.36208228
CTCGATT6706.493792E-99.26105249
GATTCCG8303.3360266E-98.30709752
CGGTACA8454.0308805E-87.75028661
TCGATTC8706.7359E-87.527998450
GGCGGTA8706.740811E-87.52769759
ACGCGAC4150.0097097457.476088557
GAATCCG9809.327778E-97.3853114
TTCTCGA9353.0569936E-87.37303347
TCACCCG12607.094059E-117.11178163
TAAATCG5450.0019550396.9561963
ATTGCCG5956.4472295E-46.95121536
ATTCCGT10001.04546416E-76.894891353
TCGTATC6552.569628E-46.8415452
GCGGTAC9654.1268686E-76.786628760
GTTACGC5650.00284881526.711840654
ACTAGGC5650.00285433426.710603710
TCTCGAT9251.4808302E-66.708005448