FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_256_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_256_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6241795
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGA1559.278792E-720.2015911
GTCGGTT5700.012.8179691
GTCCGTT8650.012.4686771
GTCGGAT6550.012.2169091
GTCGTAT6007.094059E-1111.0173511
GTCGCTT9550.010.9293091
CTCGTAT8950.010.8845331
TCGCGTA2300.00877961710.500771566
GTCGTTT10350.010.4206821
GTCGGGA8400.010.3546521
CCCGAAT7851.8189894E-1210.1937271
GTCCGGT8550.010.1729931
GTCCAAT19200.010.1475591
GGCGTTT10450.09.98802951
GTCCATT31550.09.7041641
CTCGTGT14000.09.6919561
GTACGAT5754.9669325E-89.681151
GTCGGAA9050.09.6109491
CGATTAG3956.0429862E-59.6086074
GTCGCAT4351.5082096E-59.59769251