FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_255_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_255_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5128954
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAAG2550.00154119210.81535957
GTTTCGA3956.420168E-48.7302241
GTTTAAT23950.08.4951091
TATATCG3900.0055838577.9528143
CGGTACA6806.4747746E-67.603288761
GTTTTTT69900.07.05474471
GTTGAGT13901.2732926E-116.9464941
GTTAGAC7007.700952E-56.8968771
CGGGAAT8502.8140075E-66.89687631
TAAGCGT5100.0072849196.817106217
GAACGAT9201.3458339E-66.7469441
CGAGAAT6200.00102366796.67439651
AAATCGT8301.4733612E-56.64331444
CTTAGAT13503.5106495E-106.64143661
ATTCGAG6753.7197408E-46.6374225
GGCGGTA6500.00175548646.36349259
GGGAAAT32250.06.3087711
GGTTAAT12107.659219E-86.2698881
GTTCACT20450.06.07061
GCTAAAC13105.671609E-86.05450961