FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_255_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_255_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5128954
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG2006.00503E-717.2481631
GTCGGTT4050.015.4698651
CGACTTA1808.087991E-515.3346874
GTCGAGT4152.0008883E-1114.2583691
CCCGTAT4507.6397555E-1113.1493851
GTTCGAC2651.1183318E-513.01646329
GTCCGTA6350.012.0591871
GTCGAAT4206.777918E-811.6023991
GTCGCAT4257.986637E-811.4658991
GTCCAGT26500.011.295921
GTCGCTT6850.011.1789531
GTCCGAA5652.8740033E-1011.0890181
CTCGCAT6058.185452E-1110.9311861
GTCCCGT7350.010.8920491
GTCGTTT9600.010.877251
GTCACGA7201.8189894E-1210.6355321
GTCCGCT7201.8189894E-1210.6355321
CCGTATG4006.135755E-610.3518232
GGCGTTT8600.010.1183711
GCCGTTT8950.010.11158851