FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_254_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_254_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18471329
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT22450.010.441421549
TCTCGAT24500.09.70845348
GATTCCG26850.09.24482752
CGGTACA27650.09.10030661
ATTCCGT25800.08.9529453
GGCGGTA29650.08.48664159
TTCTCGA28500.08.34500547
GCGTAGT17200.08.28684518
TCGATTC28750.08.27279950
GTCTAAG35000.07.58350144
GCGTCGT21400.07.472705419
GTTTAAT84800.07.3625161
TCACCCG37800.06.83811763
ACTAGGC23700.06.835525511
CGATTCC36150.06.770992851
ATCGACT30000.06.66351376
GCGGTAC35600.06.584048760
GTTTGAT105300.06.5188081
TTGCGTA19750.06.513076316
CTCACTA51400.06.50456247