FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_254_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_254_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18471329
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGTT17600.013.8401431
TCGACCG9900.012.19557231
TCGGTTA11350.011.5520282
GTCCGAT22350.011.2101171
GTCCGTT22550.010.8020621
GTCGGAA30200.010.7159631
TCGCGTA8550.010.49221366
GCGATCG12150.010.22110740
CGACCGT12150.010.22105132
CGCGTAA8450.010.20805967
GTCGGCT26750.010.1467281
GTCGTTT31100.010.0701821
GTCGTTA16650.09.822881
GCCTAAT23300.09.7076171
GTCCGCT23350.09.686831
GTCCGGA26100.09.5994681
GATCGCG7951.4551915E-119.54612740
GTCGAGA15250.09.3555471
GTCGCAT13450.09.3139831
GTCGGAT27100.09.2452451