FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_253_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_253_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23099691
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACA32600.09.62161761
GGCGGTA35100.08.05266259
GATTCCG29050.07.594952
ACTAGGC28700.07.566354311
CTCGATT28150.07.469810549
GTTTAAT105500.07.35635761
GTCTAAG40050.07.229661544
GCGGTAC42000.07.222065460
CGGGTTA11352.9776857E-96.98510943
TCTCGAT29500.06.894267648
TCGATTC32550.06.88333550
TTCTCGA33500.06.79094147
CGCGCTA11452.5998816E-86.62292623
CGCGGTA12201.1157681E-86.554337517
ATTCCGT34150.06.46068353
CGATACG23000.06.444362629
ATCGACT36250.06.3702066
ACACACG55050.06.324879652
TACATCG43700.06.309193
GTTAGAT59950.06.27148061