FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_253_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_253_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23099691
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG10550.015.04080931
CCCGTAT18250.013.157621
CGACCGT13400.012.61412432
GTCCGTT28250.011.7029751
GTTCGAC15050.011.68931829
GTCCGAT25950.011.2650481
GTCCGCT30150.011.1963261
CCCGAAT30300.010.681481
GTCCGGA33450.010.2998331
GTCGAGA19100.010.2034211
GTCGCGA6009.085852E-99.86027051
TCGCGTA8203.6379788E-129.67771666
GTCGAAT18800.09.6257951
TCGACGC18300.09.61336743
GTCGGTT21000.09.6116911
GTCGTTT37250.09.5293871
GTCGCTT33800.09.4724491
GTCCGCA31000.09.3176741
GTCCAGT106300.09.166761
GTCGGCT32350.09.1439911