FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_252_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_252_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5333830
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGGTA6453.0557603E-68.01606159
GTTATAT13700.07.55278541
TCTCGAT6402.3724107E-57.54067348
GTTTAAT25250.07.1031081
GCGGTAC7351.9356128E-57.03450360
GTTTATT32950.06.90867851
CTTTAAT30850.06.8199491
AATCGAA5600.00260115376.7701385
TCGATTC6753.710455E-46.638797350
GATCCAA23100.06.56498155
AATCTAC15352.3646862E-116.5115215
GTTGAAT20200.06.4884161
GACGTAC8552.2760241E-56.44980675
GGAAAAT48750.06.43833071
ACGTACC8107.4866315E-56.3826226
CTTAGAT14151.036824E-96.33757831
GGGAAAT31100.06.32151171
GTTTGAT31150.06.31136471
CCGGAAA6600.00206961046.2711011
CGGTACA7803.1658306E-46.186638461