FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_252_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_252_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5333830
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAT4450.014.8544321
TCGACCG2152.2693339E-514.4395531
CGACCGT2751.0813546E-613.79779232
GTCCGTA7600.011.9020611
GTCGGTT4104.8825314E-811.8797321
GTCGGAT5652.3646862E-1111.6995091
GTTCGAT7750.011.6716991
GTCGCTA4802.9813236E-911.5968811
GTCCGGT8700.011.5968811
GTCGGGA7650.010.9147121
GTCGTTA4854.2993634E-810.7599931
GTCCGTT6151.10958354E-1010.7483291
CCGGCAA3309.643211E-510.5426191
GTCGTAA4753.729092E-710.2540841
GTCGGCT9650.010.0946941
GCCTTAT10700.010.0795321
GCCGTAT5552.87273E-810.0297351
CCCGTAT4905.7106445E-79.9401841
GCCCTAT9000.09.6640671
GTCGAAT4703.602685E-69.6229441