FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_251_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_251_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4846115
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTCCG6251.8189894E-1211.5831252
CTCGATT6402.3646862E-1110.77221349
TCGATTC7301.8189894E-1210.38811750
ATTCCGT6901.0186341E-109.99234153
TCTCGAT6757.377821E-99.19228948
TTCTCGA6808.383722E-99.1240447
CGGTACA8851.4551915E-118.95821761
CTCGAAC3500.00208508718.8690261
GGCGGTA8607.3123374E-108.4170159
GCGGTAC9506.91216E-118.34528560
AATCGTC4150.00105365848.30542855
CGATTCC9001.7771526E-98.04383451
TACATCG9152.4647306E-97.9099273
GTTTGAT30000.07.7029141
ACGGGCG10556.4210326E-107.515100556
GGGCGGT11102.382876E-107.452822758
GTCTAAG10303.1250238E-97.362603744
TAGCATA13901.8189894E-127.19143779
TTGAATC19300.07.14456132
TTAAGCC12657.8216544E-117.08452731