FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_251_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_251_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4846115
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTT6050.014.9520661
GTCGGTA4500.014.6900871
CGACCGT3204.1936255E-712.93591332
TCGACCG3551.07627784E-712.63225331
GATCGCG2204.4452402E-412.54391640
CCGGAAA4054.1134626E-812.0269711
TCGCGAG2306.462225E-411.998528542
CGCGAGT2601.3487888E-411.94059743
CCGGTCT6650.011.5102381
GTCGGTT5157.3305273E-1011.484841
GCCGTAT4004.5382512E-711.3075011
GTTCCGT6501.8189894E-1211.2405931
GTCGGAA7500.011.1335391
GTCGAGA3451.2401315E-511.0932011
GTCGCTT6950.011.0133931
GTTCGTT9050.010.7644721
GTTCGAC3904.552974E-610.61364629
TACGACG3652.545427E-510.3949139
GCCTATT9050.010.3800271
GTCCGTA5754.5329216E-910.2864231