FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_240_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_240_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7097969
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACA10350.010.32380161
GGCGGTA11050.09.35807359
GCGGTAC12250.09.28550160
GTCTAAG12450.08.85991444
GACGTAA5959.618616E-78.6901341
ATTCTCG7403.555033E-88.3832056
ACTTGCG6755.8314436E-67.659810510
ACACACG17850.07.532135552
CGGTAAT4800.0040483817.242459319
CTTGCGT6906.414064E-56.993739611
CTAAGTA16300.06.97915446
GTTTAAT33650.06.96965841
TCTAAGT18550.06.87597445
CAATCCG6602.8276988E-46.78827764
CTCGATT7653.381588E-56.759180549
CGAGAAT7151.01471516E-46.75321
TAGCATA19850.06.59848839
CGAAACT6904.8168673E-46.49803261
ACGGGCG16603.6379788E-126.43782656
ACACGGG17700.06.427356754