FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_240_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_240_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7097969
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGA1602.7357151E-517.4047971
TCGCGTT3651.0808435E-813.2353262
GTTCGAT9250.013.1711981
TCGACCG3457.4112904E-812.99865131
CGATCGG4752.2009772E-1012.3462641
CGACCGT4205.81349E-912.32014932
TCGCGTA3104.1397925E-612.24230366
GTTCGAC3953.2745447E-812.22588929
GTCCGTT8650.012.0726911
CCCGTAT7500.012.0673261
GTCCGCT11350.011.6543131
GTCGAAA5451.5279511E-1011.4967461
GTCGCGT4904.08545E-911.3663991
CTCGTAT8600.011.3333571
GTCGGTT5956.002665E-1111.1156691
GTCGAAT5251.2012606E-810.6086391
CGCGTAA3602.1802318E-510.54198467
CCCGAAT7301.8189894E-1210.4905631
CGAATCT7251.8189894E-1210.4709033
GTCCGGA10000.010.4428781