FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_239_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_239_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7280734
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT8100.011.49032649
GATTCCG9600.010.77269852
TCTCGAT9100.010.22765348
TACGCGA3209.252461E-49.69516356
TCGATTC9950.09.35341950
TTACGCG3400.00160997229.12485855
TTCTCGA11050.09.04612347
ATTCCGT10850.08.89616453
CGATTCC12350.08.09462251
CGCAATC4855.646458E-47.81794523
AATCGTC7951.1928933E-77.8036245
AATTCGC5351.9008254E-47.73069245
TCACCCG13200.07.5715573
CGGTACA11253.2014214E-107.35355661
GTTCAAT18950.07.2818981
TTTCTCG12556.366463E-117.141201546
GTTTAAT33950.07.11298851
GTCGATT5350.00159134227.0930451
GTATAAT20050.06.8823931
CGTGGGT14555.456968E-126.870645557