FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_239_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_239_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7280734
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG4350.016.6529831
GTTCGAC4700.015.4120229
CGACCGT4900.014.7837732
TCGACGC6500.013.79818343
GTCCGCT9100.013.0015551
CCCGTAT6150.011.8823391
TTCGACC6450.011.76524630
CCGTCTA2651.6058859E-411.7191412
GCCGTAT6005.456968E-1211.5994261
GTCGTTT12900.011.3296721
GGCGTAT7250.011.03945351
TCTCGAC6900.010.99900641
GTCGGTT6058.185452E-1110.9283851
GTCGAAT6109.458745E-1110.8388081
GTCGTTA5158.94579E-910.8111161
GTCGGCT12250.010.7945681
AGTTCGA7400.010.72082628
GTCGCAT4552.05795E-710.7071631
GTCGGAT7950.010.5051411
GTCGGAA10200.010.2347891