FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_238_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_238_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14312249
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT17750.010.8753949
CGGTACA21600.010.37245161
GATTCCG19150.010.08127352
TCTCGAT20850.09.91974248
GGCGGTA21500.09.77972959
TCGATTC21000.09.684298550
GTCTAAG25900.09.18316444
GCGGTAC24600.08.82737860
ATTCCGT21950.08.32416253
TCACCCG27950.08.0147433
TTCTCGA24100.07.86632747
CGATTCC26150.07.778234551
ACTAGGC24900.07.613593611
ACGGGCG30000.07.58414656
GTAGTCG21300.07.444957757
ACACACG33250.07.257758652
TCGTTGA26800.07.20359652
CGGGAAT32700.07.17266371
CCGGACA28850.07.16838212
TACATCG27950.07.1516173