FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_238_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_238_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14312249
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGCT17500.013.124841
TCGACCG7250.012.84696431
CGACCGT7700.012.54417432
GTCGGTT12800.012.5065051
GTCGCTT17750.011.763621
CCGTCTA4955.498805E-911.153262
GTCCGAT21400.011.058171
GTTCGAC9800.010.91131329
GTCGTTT23300.010.9032281
GCCCGAT16750.010.5960381
GTCGAGA9500.010.2570511
GTCCGTT19000.010.2570511
CCCGTAT11250.010.2082091
CCGGCTA15750.09.63677537
GTTCGAT29050.09.5836721
GTCCAGT65800.09.4670631
GTCGGAA26100.09.4668611
TCGCGTA5503.046407E-79.41016966
ATCGTCG22750.09.40087636
GTCGGGT21900.09.3755341