FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_237_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_237_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14340653
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT18300.09.60686449
GATTCCG21300.09.22507552
CGGTACA23550.08.92797661
GGCGGTA24050.08.88595959
TCTCGAT21000.08.53584748
GCGGTAC27450.08.41306360
ACTAGGC27000.08.29827711
CCGCTTA18800.08.25143153
CAACCGA23050.08.2233984
ACCCATG62900.08.0546926
TCGATTC23650.08.016335550
GTCTAAG28000.07.878995444
TCAACCG22000.07.8326173
CGATACG21600.07.81955729
CTAACTT64200.07.731562629
CGCTTAT21000.07.71530754
CGGGAAT36400.07.67544841
TCACCCG28750.07.67187833
AACCCAT80950.07.66368965
ATTCCGT22950.07.660588753