FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_237_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_237_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14340653
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG7700.020.159831
CGACCGT8700.018.63558432
GTTCGAC10350.016.6638729
GTCGGTT12400.013.4733361
TCGACGC13450.013.33670343
TTCGACC12200.013.00601530
CCCGTAT11450.012.4633261
TAGACGC20000.011.72901339
AAGTTCG13750.011.28881827
AGTTCGA14500.011.18068728
GTCGGAA26500.010.901541
TCTCGAC14750.010.75834341
GTCGTTT24750.010.687941
GTCCGAA14150.010.5771261
TCGCGTA6251.7462298E-1010.4890266
GTCCGTT18400.010.4040041
CGACGAA21950.010.37093319
CGCGCAA2350.00965004710.3677811
GTCGCTT17500.010.342391
CGATCGG9350.010.3306141