FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_236_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_236_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9954987
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG38000.053.5092623
CGTATGC42100.050.715681
ATGCCGT41850.047.433764
CCGTCTT41300.041.5592967
TGCCGTC49100.039.8002785
GCCGTCT45500.039.4648866
GTATGCC55950.037.644732
CGTCTTC59150.027.7940968
GTCTTCT71800.017.3300579
CTCGATT9950.010.39348749
TCGATTC13100.09.47290950
TCTTCTG121150.09.38889110
CTTCTGC112350.09.23458211
GATTCCG13450.09.2271952
TACGCGA3804.3792377E-49.07164556
TCTCGAT12400.08.61793248
CGCGTTA5301.702935E-47.80452858
GCGTATA5200.00118725347.2918439
CGATTCC17150.07.2366351
TTCTCGA15900.06.937044647