FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_236_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_236_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9954987
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4850.020.6315523
GTCGCGA2202.5991689E-514.2335451
CGTATGC8000.013.4823311
CGCGTAT1550.00767473913.4683021
GTCGGAT11000.012.65204051
TCGACCG5750.012.59853731
CGCGTAA3651.5420846E-712.28877667
TCGCGTA3451.0405074E-612.0010866
CGACCGT6301.8189894E-1211.49866532
GTCGCAT8000.011.3077611
GTTCGAT11700.011.3003271
GTCGGAA13400.011.1649541
GTCGCTT14550.010.9998841
GTCCGTT10350.010.7572911
GTCGGCT16650.010.4483821
CCCGATT7700.010.3927481
TCGACGC8350.010.32814343
GTCCGGT15700.010.194161
GCCGTAT10350.010.084961
CCCGTAT10700.010.0802481