FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_235_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_235_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18631821
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACA25750.08.03158361
CTCGATT19800.07.83438249
GGCGGTA26400.07.703478359
TCGATTC24200.06.979347750
GATTCCG24250.06.823767752
GCGGTAC31800.06.72041960
GTTTTTT246200.06.48630861
TTCTCGA27450.06.40408847
GTTAAAT88000.06.1534921
GTTTAAT87350.06.0808251
TCTCGAT24450.06.062433748
GTTTATT123450.06.00692271
GTCTAAG34450.06.00346544
CGGGAAT28850.05.97762731
GTTTTAT143650.05.90655371
GGAAAAT175850.05.9037611
GTTGAAT70800.05.8946331
GGGAAAT114400.05.81883431
GTTTAGT61800.05.6926751
TAGCATA49100.05.5457759