FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_235_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_235_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18631821
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG9700.014.93645131
GTCGGTT16850.013.426051
GTTCGAC11000.012.85663229
CGACCGT11550.012.24536532
GTCGCTT26600.011.9067871
GTCCGCT22550.011.5757611
GTCGTAT19400.010.9436681
GTCGCAA8900.010.5586551
GTCGGAT20650.010.4497631
GTCGGCA35100.09.8166411
GTCGCAT15800.09.6923791
CCCGAAT21200.09.6861461
TTCGACC15400.09.63127530
GTCCAGT94200.09.5693771
GTCGGTA18950.09.5505631
TCGACGC15900.09.54621543
CCGGAAA15350.09.5230421
GTCGCGT9900.09.4921251
GTCGTTT31650.09.0172671
GTCGTAA16600.09.0156121