FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_234_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_234_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19762688
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT23450.010.28991349
TCTCGAT25050.09.90789348
CGGTACA29500.09.69799261
TCGATTC28550.09.1758350
GATTCCG28250.09.15234452
ATTCCGT27900.09.14359753
GGCGGTA32850.08.60425159
TTCTCGA29150.08.27721447
GCGGTAC37050.07.814900460
TCACCCG36300.07.50038533
GTCTAAG38300.07.379979644
CGATTCC36450.07.377096751
ACTAGGC26700.07.10057810
CGATACG20950.07.07491529
GTAGTCG18550.07.061702757
CGGGAAT34300.06.73712641
TTTCTCG40400.06.569908646
ACGGGCG47100.06.513866456
GAATCGT33350.06.51040274
ACACACG48750.06.505830352