FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_234_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_234_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19762688
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG10750.016.04446631
CGACCGT13000.013.7982432
GTCGCAT14100.013.3272891
GTTCGAC15350.011.9099929
GTCCGTT23100.011.9009681
GTCGGCT30100.010.8674711
GTCGGTT15750.010.6054181
GTCGCTT26550.010.4855831
CCCGAAT22350.010.2762241
TTCGACC17750.010.10529330
GTCCGCT24000.09.8597251
TCGACGC19000.09.80406343
CCCGTAT16350.09.7905531
GTCGAAT16900.09.4719261
GTCGCAA9950.09.4429531
AGTTCGA19450.09.39930528
GTCCGGA26550.09.0438161
GTCCGTA31500.08.9483211
GCCTGAT64000.08.9172521
GTCGGAA30850.08.9112591