FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_233_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_233_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8229766
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACA12800.011.31004761
TCGCGAA3501.6125152E-510.83361733
ATCGCGA4108.341407E-610.0886532
GGCGGTA14050.09.81351659
ACGCGAT4058.02782E-59.36238658
GCGGTAC15800.08.72641760
TGACGCG9559.094947E-128.66334856
CTCGATT9952.1827873E-118.3148249
GTCTAAG15550.08.20215744
TCGATTC11751.2732926E-117.62739850
GACGTAA5452.35063E-47.58994941
CGCGAAG7302.174167E-67.55506234
GAATCCG13500.07.4031314
ACTAGGC11303.5288394E-107.32096711
TCTCGAT11505.18412E-107.19412748
TTCTCGA13152.5465852E-117.077484647
GATTCCG11252.4665496E-97.04800852
GGGCGGT20550.07.04508658
ACGGGCG20550.06.877805756
TCGTTGA10604.184767E-86.829745352