FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_233_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_233_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8229766
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG4250.017.04461131
CGACCGT4950.015.33113132
GTCGAGA5800.014.9930911
GTCGGTT7750.013.4647631
GTCCGTT10400.012.0406061
GTTCGAC6500.011.67453729
GGCGTAT9850.011.6535131
GTCGGCT11800.011.4963971
GTCCGAA8650.011.259551
GTCGGGT12100.011.2113621
GTCCGGA12350.010.9844121
GCCCTAT14150.010.8162161
GCCGATT8500.010.6398031
TCGACGC7201.8189894E-1210.54053743
GTCGCCT8350.010.4143621
GTCCGAT10400.010.3682991
CCGGCTA6453.110472E-1010.16108437
GTCCAGT38550.010.1058481
GTCGTTA5251.376211E-79.9382771
CCGGTAT9850.09.8878291