FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_220_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_220_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5422399
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGC2852.3802702E-512.0937497
CGTAACG2903.7424132E-410.697206530
CTCGATT5959.701034E-89.26959949
GATTCCG7757.736526E-88.00661252
TCTCGAT7453.512887E-77.865938748
ATTCCGT8809.933501E-97.83475253
CCGCTAG4000.0069929687.75576165
CGCTAGA4500.00233962787.66001166
TCGATTC8554.0244595E-77.25678550
TAATCCA16650.07.244624
CGATTCC9655.4509655E-87.144644751
GTTTAAT24800.06.95345451
GAATCGT6552.5759698E-46.84012174
GTTAGAT14152.1827873E-116.82470461
TCACCCG10201.5080332E-76.7575863
CGGGAAT7905.248889E-56.54856971
AGAATCG7401.6037593E-46.5201583
CGTAAAC6350.00133995516.51763151
GTCTAAG9552.5029112E-66.49703244
CCGAGTT6955.2582985E-46.4512051