FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_220_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_220_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5422399
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTA1503.5243717E-416.1022662
GTCGGTT4002.4738256E-913.0475231
CCCGTAT5450.012.7682181
CTCGAAT7600.012.360811
GGCGTTT8050.011.6698331
GTCGGAA7200.011.5977981
GTCGGAT5753.274181E-1111.4969461
GTCCGGT7750.011.2236751
TCGACCG3702.4222118E-611.18766931
CGACCGT3752.8456616E-611.03849932
CCCGAAT7250.011.0379041
GTCCGTT6950.011.0137361
GTCCGAT5703.3651304E-1010.9873871
GTCGTTA4501.7701859E-710.8246111
GTTCGAC4158.157731E-710.80536129
CCCGGAT11100.010.6574341
AAGTTCG5301.6041668E-810.413197527
CCGCTAT4057.1185696E-610.2236613
CTCGTAT7503.6379788E-1210.2060611
TCCGCTA3854.5599347E-59.8585312