FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_219_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_219_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4771256
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT5501.6370905E-1111.90841349
GATTCCG6503.8544385E-99.54621552
TTCTCGA7051.5807018E-99.28976447
TCGATTC6706.389564E-88.74600650
TCTCGAT7001.293738E-78.37170348
ATTCCGT7706.9361704E-88.05849453
CCGGACA8202.366869E-87.986596612
CGGTACA6503.4100121E-67.95451261
ACCCGGC11507.2759576E-127.79262645
ATCGACT6452.6348724E-57.4812146
GTTGAAT18350.07.3318711
CCCGGAC9504.0961822E-87.256595611
GTTTATT30650.07.0908181
TCACCCG9452.871111E-76.929663
CGATTCC8502.8281647E-66.894633351
TACATCG9957.01928E-76.58143573
GGAAAAT43550.06.4162781
AATCCAC14955.7298166E-106.2248795
CGAAAAT6150.00685492676.1702651
GTTTAAT23450.06.0315271