FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_219_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_219_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4771256
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG2608.913048E-1118.57436231
CGTCTAG1300.0025259515.920547536
GTCGCGT2452.637753E-715.6306721
GTTCGAC3151.364242E-915.33057629
GTCCGCT5050.015.1663961
CGACCGT3202.7659553E-814.01369132
CCCGATT3659.540599E-913.3532281
GTCGGTT3456.5731E-813.1182321
TCCGCTA1850.001725162413.0556742
CTCGTAA5004.4383341E-1011.8366821
TCGACGC3853.0911178E-711.64786643
CCCGTAT4802.9504008E-911.604591
TCTCGAC4904.7039066E-911.26434241
AAGTTCG4753.5901394E-810.89266427
GTCCGTA5804.5110937E-1010.8042741
GTCGCAT3551.6902779E-510.7873661
GTCGAAT3004.667848E-410.4441321
GTCGTAA4005.518621E-610.4441321
TTCGACC4301.2880228E-610.42835730
GTCCGCA6704.7293724E-1110.3921711