FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_218_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_218_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15284348
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTCCG19650.010.175652552
CTCGATT19750.09.77431849
TCTCGAT19600.09.67324548
TCGATTC21200.09.43047450
ACTAGGC19500.09.19176211
ATTCCGT21650.08.75793453
TTCTCGA23400.08.54379347
CGATACG15950.07.99612529
TCACCCG30200.07.9883253
CGATTCC27000.07.6610351
CGGTACA20950.07.23931161
CGCGCTA7151.3147528E-57.231328523
TTGCGTA12202.0918378E-107.124228516
TCGTTGA21950.07.067646552
CGTGGGT31850.07.035386657
GTTTAAT72100.07.03127151
GCGTAGT12101.3023964E-96.89566818
CCGGACA30300.06.711740512
CCGCTAG9251.4825873E-66.707765665
TTTCTCG31400.06.69664446