FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_218_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_218_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15284348
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG7800.016.36365131
CGACCGT8900.014.34117632
GTCCGTT19500.012.4885571
GTCGGTT12500.012.2459221
GTCCGAT21900.012.0730871
GTTCGAC11350.011.24498929
CGCGTAA6251.7462298E-1010.48884567
GTCGCAT10400.010.3699621
GTCGTTT25600.010.1922511
TCGCGTA6807.6397555E-1110.14787666
GTCGCTT19850.09.9899461
TCTCGAC13500.09.96590441
GTCCGCT20300.09.9398731
CGATCGG9850.09.80635641
TTCGACC13100.09.74279630
GTCGCTA14750.09.6703171
TCGACGC13350.09.56069143
GTCCGAA16200.09.4490141
CCGGAAA12250.09.3718791
GCCCGAT16000.09.3496921