FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_217_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_217_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20473828
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT26850.011.42621549
TCTCGAT28850.010.39513548
GATTCCG32250.09.19306852
ATTCCGT31750.09.12070453
CGGTACA33400.08.97837361
TCGATTC33550.08.83573150
TTCTCGA33250.08.81152447
GGCGGTA35150.08.3354359
CGATTCC39700.07.989015651
GTAGTCG21200.07.805186757
CGGGAAT36650.07.6229761
CGCTAGT10102.1063897E-97.50843633
GTCTAAG39250.07.4648544
TCACCCG42950.07.1415123
GCGGTAC43300.07.084893760
GAATCGT38500.07.0717964
AATCGTC36100.07.0649845
ACACACG48450.06.973069752
GTTTAAT95450.06.9380721
CCGGACA44600.06.878215312