FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_217_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_217_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20473828
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG12700.018.7423831
CGACCGT13950.017.06295432
GTTCGAC16400.014.51320729
TCGACGC19100.013.18427243
TTCGACC18800.013.02742830
GTCGGTT17400.012.5968151
GTCGCGA5651.8189894E-1212.315471
AGTTCGA22200.011.34283728
CCCGAAT22900.011.2425731
GTCCGTT24750.010.8239311
GTCGTTT35100.010.8041071
CCCGTAT17750.010.7803741
GTCCGAT26550.010.7453061
GTCCGGT32850.010.6968391
CGATCGG15000.010.57892941
AAGTTCG22250.010.38715527
GTCGCTT26750.010.2747851
GTCCGCT25000.010.1590321
GCCGTAT21250.010.1508461
GTCGAGA18200.09.9403441