FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_216_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_216_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17277003
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACA26400.09.79231161
GGCGGTA24900.09.69031359
CTCGATT21050.09.00699549
GATTCCG23150.08.93500352
TCTCGAT23250.08.30298748
GCGGTAC29400.08.089787560
TCGATTC25000.07.85916750
ATTCCGT25700.07.780174353
GTCTAAG32100.07.731729544
TCACCCG33200.07.5776743
TTCTCGA24300.07.3762647
ACGGGCG35550.07.27276956
CCCGGAC32750.07.26215611
TTACGCG7452.3363034E-56.940857455
CCGGACA33350.06.92471312
TAGCATA44550.06.88592969
ACACACG41700.06.69643152
GTTAAAT84100.06.68458941
GTTTAAT80100.06.5017091
GTTTGAT103250.06.4468921