FastQCFastQC Report
Thu 20 Oct 2016
WTCHG_320658_216_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_320658_216_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17277003
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG9400.015.77988131
GTCCGTT20600.013.8543471
CGACCGT11150.013.30321932
GTTCGAC12250.012.67113929
GTCGGTT16650.012.33324051
TTCGACC13100.011.84896630
GTCCGGT24000.011.7466271
GTCCGAT17000.011.6698531
GTCGGAT19950.011.5143781
TCGACGC15300.011.04759643
GTCGCGA3802.9979656E-610.9909961
GTCGCTT24450.010.9610281
CCCGAAT18300.010.8408471
GTCCGGA20850.010.5165651
GTCCGCT22100.010.39421
CCCGTAT17150.010.35012151
GTCGCAT13700.010.1619921
GTCGTAT18600.010.1046271
AAGTTCG16100.010.0694327
CTCGTAT22850.010.0530341